PIK3C3 Knockout cell line (VERO C1008 (E6))

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIK3C3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the VERO C1008 (E6) cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIK3C3 gene knockout PIK3C3 Knockout cell line (VERO C1008 (E6)) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Catalog#

YKO-R021

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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PIK3C3 Knockout cell line (VERO C1008 (E6))

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